Useful life of frozen semen increased
The features of a new diluent named “Poly-Crio" are possible thanks to a recently discovered polyphenol in a variety of a plant of the annonaceae family, a plant which has been scarcely researched in Colombia.Medellín, 24 de mayo de 2016 — Agencia de Noticias UN-
This diluent increases the useful life of frozen semen in different animals. Photos: Giovanni Restrepo
The features of the “Poly-Crio” are possible thanks to discovering a polyphenol in a variety of a plant of the annonaceae family.
Initial tests were carried out on equine semen, which is often vulnerable to oxidative stress. When freezing sperm cells with the discovered diluent Universidad Nacional de Colombia (UNal) in Medellín researchers proved a 22 to 25% viability increase of the aforementioned cells.
“A very important parameter is spermatic mobility, therefore we observed how many of these cells preserved these mobility patterns,” said UNal Faculty of Agrarian Sciences Professor Giovanni Restrepo.
Part of the frozen and unfrozen material was used to inseminate mares which later produced viable foals.
These results show advancements in reproductive biotechnology, artificial insemination, and embryo transference which translates in better reproductive rates and a positive impact on animal productivity.
With the encouraging results with equine semen, tests were escalated for cattle, swine and human semen with very promising results. This demonstrates the discovery has ample applications and uses.
The patent pending diluent offers long-term frozen sperm cell viability rates above 90%, which is superior to most diluents offered in the market.
In most animals, more than 60% of the sperm preserve their integrity and mobility when they are used for seminal freezing.
Stress impacts semen
The sperm used for artificial insemination in different animal species can undergo stress levels due to temperature changes and thus have a bearing on the fertilization capacity loss and reduction of birth rates of viable broods.
“In this particular case, sperm cell stress translates into alteration of the plasmatic membrane due to a lipid peroxidation process which can ultimately cause harm to the DNA, and structural and functional proteins,” said Restrepo.
Diminishing the stress risks which can occur during the cooling, freezing and post-freezing processes to warrant the useful life and semen quality is the most recent research carried out by Restrepo and Faculty of Sciences Professor Benjamín Rojano. By means of an interdisciplinary project they developed a semen diluent which they named “Poly-Crio”.
The process is an achievement of the Politécnico Colombiano Jaime Isaza Cadavid and UNal and recently support from Tecnnova, with the hope of “Not leaving the research project on the shelf but take it to the next level,” said Rojano.(Por: Fin/SLGS/DMH/APBL